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Can Mepis or Knoppix be used in a small business with 23 desktops? What would be their limitations, if any? Would there be another choice that's free?
Either Mepis or Knoppix could be used in a small business. I assume you're interested in using a LiveCD Linux distro to enable everyone to gain some familiarity with Linux to see if it will work for the organization. The big advantage of LiveCD distros is that they enable you to work with Linux without having to do an install and without making any permanent changes to the filesystem. Their disadvantage is the flip side of the impermanence: you can't permanently configure the system(s) to work in a particular way that makes sense for your organization. There are other LiveCD choices, but the time spent finding and assessing them probably will outweigh the advantage gained by beginning to work with one of the ones you've identified already.